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Image Search Results
Journal: International Journal of Molecular Medicine
Article Title: Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro
doi: 10.3892/ijmm.2018.3431
Figure Lengend Snippet: Immunohistochemical staining. Stronger positive expression (brown-yellow color) of Col II and weaker expression of Col I was observed in the nHC group compared with that in the HC and pure collagen groups, indicating that the chondrcocyte phenotype and biological functions could be maintained effectively when cells were cultured on nHC film for a long time period. HC, hydroxyapatite/collagen; nHC, nano-HC; calcein-AM/PI, acetoxymethyl/propidium iodide; TGF-β, transforming growth factor-β; COL-I, collagen type I; COL-II, collagen type II.
Article Snippet: Anti-COLIA1 and -
Techniques: Immunohistochemical staining, Staining, Expressing, Cell Culture
Journal: International Journal of Molecular Medicine
Article Title: Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro
doi: 10.3892/ijmm.2018.3431
Figure Lengend Snippet: RT-qPCR analysis. Cartilage matrix relative gene expression was analyzed by RT-qPCR. The chondrocytes were seeded on the nHC, HC and pure collagen films and cultured for 6 days. Gene expression in cells relative to the control group was calculated by the 2 −ΔΔCq method using the β-actin gene as the internal control. The data are reported as the mean ± standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the collagen-based groups; # P<0.05, ## P<0.01 and ### P<0.001 for comparisons between the collagen-based groups and the TGF-β group. HC, hydroxyapatite/collagen; nHC, nano-HC; calcein-AM/PI, acetoxymethyl/propidium iodide; TGF-β, transforming growth factor-β; COL-I, collagen type I; COL-II, collagen type II; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Article Snippet: Anti-COLIA1 and -
Techniques: Quantitative RT-PCR, Gene Expression, Cell Culture, Control, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction